Generation of Multiple mRNA Fingerprints Using Fluorescence-Based Differential Display and an Automated DNA Sequencer
نویسندگان
چکیده
منابع مشابه
Generation of multiple mRNA fingerprints using fluorescence-based differential display and an automated DNA sequencer.
Differential display is a method for the survey, analysis and comparison of gene expression in eukaryotic cells and tissues. Differential display involves isolation of high-quality nondegraded RNA, selective reverse transcription of polyadenylated mRNA using specific anchored oligopolydeoxythymidine [oligo(dT)] primers, and the subsequent PCR amplification of the cDNA with the same oligo(dT), a...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97223rr05